RAbs are a type of antibody fragments produced using recombinant antibody coding genes that are obtained from library display. No immunogenicity issue (for naïve libraries) Possible to directly screen human libraries However, phage display is more expensive compared with hybridoma, and it is possible to result in a lower affinity when panning naïve libraries.Įasy to screen a large diversity of clones Unlike hybridoma development, when a naive library is available, the process can be very fast and usually only needs a few weeks to screen a greater diversity of antibodies. Subsequently, the sequence is obtained easily that facilitates further engineering and recombinant antibody production. Libraries can be generated from any animal to directly screen antibodies. Thus, a library of naïve or immune phage is constituted and can be used to detect an antigen-antibody interaction of interest through screening methods. After the display process, a large number of antibodies are produced rapidly. The phage infects Escherichia coli and then continues to display new phage in the host cell. Simply, the process refers to a gene sequence coding for a particular antibody that is integrated into the DNA sequence of a filamentous bacteriophage, which can be expressed on the surface of the bacteriophage capsid. Phage display method was created by Smith in 1985. Therefore, hybridoma development is being progressively replaced by faster, more appropriate techniques for biotherapeutic development such as recombinant antibody production using phage display technology. Secondly, the mouse origin of antibodies means further humanization for therapeutic purposes, which significantly leads to additional costs. Firstly, the antibody development using hybridoma is very time-consuming, which will take on average between 6 and 8 months to obtain a reasonable amount of monoclonal antibodies (against a few weeks for phage display). However, it is worth mentioning that hybridomas also have some significant drawbacks. Hybridomas technology display unparalleled advantages that make it also used for therapeutic antibody development. Importantly, the integration of the mammalian origin of the cells and in vivo post-translational modifications significantly decrease the risk of aggregation or recognition failures. Hybridoma development represents one of the most traditional methods for the generation of monoclonal antibodies, which can produce highly sensitive binders and make them particularly adapted for assay development. Antibody of interest is produced by the immortal B cells, and then the best clones are screened to obtain monoclonal antibodies with the desired antigen affinity. This technology is widely used for the antibody production based on the fusion of mouse immunized B spleen cells with myeloma cells. Hybridoma was first developed in 1975 by George Köhler and César Milstein. We believe our off-the-shelf products and high-quality services will support your needs.Īntibody Production Methods: Hybridoma vs Phage Display Currently, rAb can meet all applications in which traditional mAbs are used and present inherent advantages over their traditional animal-derived counterparts.Ĭreative Biolabs has a world-class technical team to provide the most comprehensive list of rAb products and customized rAb development services for clients. rAb is produced using antibody genes (screening by phage display) in a laboratory or taken from human cells, with completely no need for animals. Fortunately, an alternative to animal-based mAbs appears, namely recombinant antibodies (rAb). However, the mouse ascites method of mAb production (such as Hybridoma) is significantly limited due to the substantial pain and distress involved, and raised serious animal welfare concerns. Monoclonal antibodies (mAbs) are widely used in biomedical research and medicine to fight, diagnose and research diseases and to develop and test new drugs.
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